Evaluating the Angiogenic and Mechanical Properties of Hydrogels and Physical Constructs Derived from Spinal Cord Meninges Extracellular Matrix

Abstract The vasculature is an integral unit of the tissue microenvironment due to providing nutrients and oxygen surrounding cells. Therefore, pro-angiogenic biomaterials have potential improve success a wide range medical therapies, including engineering, wound healing, drug delivery. Herein, we decellularized bovine spinal cord meninges with Triton X-100 digested them pepsin obtain hydrogel (MeninGEL). cryogel form MeninGEL was also prepared by lyophilization process (named as MeninRIX). DNA content analysis showed that nuclear significantly reduced 98.6% after decellularization process. Furthermore, effect on extracellular matrix components investigated glycosaminoglycan (GAG) hydroxyproline (HYP) analyses. Tensile, compression, suture retention tests were performed elucidate mechanical properties. physiological degradation behavior bioscaffolds hydrolytically. Both MeninRIX good biocompatibility properties, proved Chick Chorioallantoic Membrane (CAM) assay. Moreover, SEM histological analyses indicated cellular migration, attachment, dynamism bioscaffolds’ surfaces. On basis these data, are structures adequate which makes promising candidates for soft regenerative medicine applications.